Control of mitochondrial network size, topology and function in budding yeast cells.
The long-term goal of my lab is to understand the spatial and dynamic organization of the cell at all levels of complexity:
-How is the 3-dimensional geometry (size, shape, position, dynamics) of organelles and intracellular structures generated at the molecular level, regulated system-wide to respond to the needs of the cell, and integrated to organize the cell?
-How does improper internal organization contribute to the cellular basis of disease?
Model system and approach:
The geometry of mitochondrial networks is vital to their cellular functions, regulated by the cell, and visualizable by light microscopy. Working in budding yeast allows us to integrate new knowledge of the control of mitochondrial network morphology with that of other cellular processes such as cell cycle and cellular metabolism due to the phenomenal genetic techniques and systems-level analysis possible in this organism.
We take an interdisciplinary approach, combining 3D microscopy and image processing with molecular genetics and computational methods.Continue Reading →