Wenqi Wang, Ph.D.
3208 Natural Sciences I
University of California Irvine
Irvine, CA 92697
Phone (office): 949-824-4888
The Wang lab
The central question the Wang lab is interested in investigating is what are the signaling networks underlying tissue homeostasis and organ size control as well as the role of their dysregulation in tumorigenesis. The recognition in the past decade that the Hippo pathway is a crucial signaling pathway in organ size control, allowed us to take the first step to achieve this long-term goal.
We are majorly working on the two areas in the lab:
- Dissecting and targeting the Hippo pathway for cancer treatment.
Our previous proteomic analysis established the protein-protein interaction network for the human Hippo pathway, which helped to uncover a number of potential novel Hippo regulators. We are characterizing the cellular functions of these regulators, and examining their roles in organ size control and tumorigenesis. Since Hippo pathway is a crucial tumor suppressor pathway, we also hope to explore the therapeutic potentials by utilizing the Hippo pathway for cancer therapy.
- Defining the protein-protein interaction landscape involved in organ size control and tissue homeostasis.
Coordination of key signaling pathways required for cell proliferation and survival may contribute to the elegant mechanisms underlying organ size control and tissue homeostasis. To achieve a comprehensive understanding of this process, we have defined the protein-protein interaction networks for several key signaling pathways/protein families related to growth control and cancer development, especially how they crosstalk with the Hippo pathway by utilizing the proteomic approach. We expect that these studies will also generate additional clues to help us unravel the molecular basis of the Hippo pathway in organ size control and cancer prevention.
Recent Publications (Complete list of published work: https://www.ncbi.nlm.nih.gov/sites/myncbi/1fcEFO6Btur5-/bibliography/52094817/public/?sort=date&direction= descending)
- Han H, Qi R, Zhou JJ, Ta AP, Yang B, Nakaoka HJ, Seo G, Guan KL, Luo R, Wang W. Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Molecular Cell. 2018 Sep 13. pii: S1097-2765(18)30698-1. doi: 10.1016/j.molcel.2018.08.038. [Epub ahead of print] PMID: 30293781
- Sang LJ, Ju HQ, Liu GP, Tian T, Ma GL, Lu YX, Liu ZX, Pan RL, Li RH, Piao HL, Marks JR, Yang LJ, Yan Q, Wang W, Shao J, Zhou Y, Zhou T, Lin A. LncRNA CamK-A Regulates Ca2+-Signaling-Mediated Tumor Microenvironment Remodeling. Molecular Cell. 2018 Oct 4;72(1):71-83.e7. doi: 10.1016/j.molcel.2018.08.014. Epub 2018 Sep 13.
- Han H, Yang B, Nakaoka HJ, Yang J, Zhao Y, Le Nguyen K, Bishara AT, Mandalia TK, Wang W. Hippo signaling dysfunction induces cancer cell addiction to YAP. Oncogene. 2018 Aug 1. doi: 10.1038/s41388-018-0419-5.
- Yao F, Zhou Z, Kim J, Hang Q, Xiao Z, Ton BN, Chang L, Liu N, Zeng L, Wang W, Wang Y, Zhang P, Hu X, Su X, Liang H, Sun Y, Ma L. SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity. Nat Communications. 2018 Jun 11;9(1):2269. doi: 10.1038/s41467-018-04620-y.
- Zheng X, Han H, Liu GP, Ma YX, Pan RL, Sang LJ, Li RH, Yang LJ, Marks JR, Wang W#, Lin A#. LncRNA wires up Hippo and Hedgehog signaling to reprogramme glucose metabolism. The EMBO Journal. 2017; 36(22):3325-3335. (# co-corresponding authors)
- Li X, Wang W, Chen J. Recent progress in mass spectrometry proteomics for biomedical research. Science China. Life sciences. 2017; 60(10):1093-1113.
- Han H, Yang B, Wang W. Angiomotin-like 2 interacts with and negatively regulates AKT. Oncogene. 2017; 36(32):4662-4669.
- Li X, Han H, Zhou MT, Yang B, Ta AP, Li N, Chen J#, Wang W#. Proteomic Analysis of the Human Tankyrase Protein Interaction Network Reveals Its Role in Pexophagy. Cell Reports. 2017; 20(3):737-749. (# co-corresponding authors)
- Li X, Gao M, Choi JM, Kim BJ, Zhou MT, Chen Z, Jain AN, Jung SY, Yuan J, Wang W#, Wang Y#, Chen J#. Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-coupled Affinity Purification/Mass Spectrometry Analysis Revealed a Novel Role of Neurofibromin in mTOR Signaling. Molecular & Cellular Proteomics : MCP. 2017; 16(4):594-607. (# co-corresponding authors)
- Li X, Tran KM, Aziz KE, Sorokin AV, Chen J#, Wang W#. Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family. Molecular & Cellular Proteomics : MCP. 2016; 15(9):3030-44. (# co-corresponding authors)
- Yi J, Lu L, Yanger K, Wang W, Sohn BH, Stanger BZ, Zhang M, Martin JF, Ajani JA, Chen J, Lee JS, Song S, Johnson RL. Large tumor suppressor homologs 1 and 2 regulate mouse liver progenitor cell proliferation and maturation through antagonism of the coactivators YAP and TAZ. Hepatology. 2016; 64(5):1757-1772.
- Li X*, Wang W*, Xi Y, Gao M, Tran M, Aziz KE, Qin J, Li W, Chen J. FOXR2 Interacts with MYC to Promote Its Transcriptional Activities and Tumorigenesis. Cell Reports. 2016; 16(2):487-497. (* co-first authors)
- Chen Z, Tran M, Tang M, Wang W, Gong Z, Chen J. Proteomic Analysis Reveals a Novel Mutator S (MutS) Partner Involved in Mismatch Repair Pathway. Molecular & Cellular Proteomics : MCP. 2016; 15(4):1299-308.
- Liu X, Xiao ZD, Han L, Zhang J, Lee SW, Wang W, Lee H, Zhuang L, Chen J, Lin HK, Wang J, Liang H, Gan B. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nature Cell Biology. 2016; 18(4):431-42.
- Jun S, Jung YS, Suh HN, Wang W, Kim MJ, Oh YS, Lien EM, Shen X, Matsumoto Y, McCrea PD, Li L, Chen J, Park JI. LIG4 mediates Wnt signalling-induced radioresistance. Nature Communications. 2016; 7:10994.
- Wang X, Jung YS, Jun S, Lee S, Wang W, Schneider A, Sun Oh Y, Lin SH, Park BJ, Chen J, Keyomarsi K, Park JI. PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness. Nature Communications. 2016; 7:10633.
- Wang W, Li N, Li X, Tran MK, Han X, Chen J. Tankyrase Inhibitors Target YAP by Stabilizing Angiomotin Family Proteins. Cell Reports. 2015; 13(3):524-532.
- Wang W, Xiao ZD, Li X, Aziz KE, Gan B, Johnson RL, Chen J. AMPK modulates Hippo pathway activity to regulate energy homeostasis. Nature Cell Biology. 2015; 17(4):490-9.
- Wang W*, Li X*, Lee M, Jun S, Aziz KE, Feng L, Tran MK, Li N, McCrea PD, Park JI, Chen J. FOXKs promote Wnt/β-catenin signaling by translocating DVL into the nucleus. Developmental Cell. 2015; 32(6):707-18. (* co-first authors)
- Li X*, Wang W*, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J. Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. Molecular Systems Biology. 2015; 11(1):775. (* co-first authors)
- Li N, Zhang Y, Han X, Liang K, Wang J, Feng L, Wang W, Songyang Z, Lin C, Yang L, Yu Y, Chen J. Poly-ADP ribosylation of PTEN by tankyrases promotes PTEN degradation and tumor growth. Genes & Development. 2015; 29(2):157-70.
- Wang W, Li X, Chen J. Energy crisis and the Hippo pathway. Cell Cycle. 2015; 14(13):1995-6.
- Wang W*, Li X*, Huang J, Feng L, Dolinta KG, Chen J. Defining the protein-protein interaction network of the human hippo pathway. Molecular & Cellular Proteomics : MCP. 2014; 13(1):119-31. (* co-first authors)
- Zhang J, Zhang P, Wei Y, Piao HL, Wang W, Maddika S, Wang M, Chen D, Sun Y, Hung MC, Chen J, Ma L. Deubiquitylation and stabilization of PTEN by USP13. Nature Cell Biology. 2013; 15(12):1486-1494.
- Fong KW, Li Y, Wang W, Ma W, Li K, Qi RZ, Liu D, Songyang Z, Chen J. Whole-genome screening identifies proteins localized to distinct nuclear bodies. Journal of Cell Biology. 2013; 203(1):149-64
- Wang W, Huang J, Wang X, Yuan J, Li X, Feng L, Park JI, Chen J. PTPN14 is required for the density-dependent control of YAP1. Genes & Development. 2012; 26(17):1959-71.
- Wang W, Huang J, Chen J. Angiomotin-like proteins associate with and negatively regulate YAP1. Journal of Biological Chemistry. 2011; 286(6):4364-70.