HomeFacultyScott Atwood

Scott Atwood

Scott Atwood, Ph.D.
Assistant Professoratwood

4238 McGaugh Hall
University of California Irvine
Irvine, CA 92697-2300
Tel: (949) 824-4346
Fax: (949) 824-4709
Email: satwood@uci.edu
Twitter: @atwoodlab
Website: http://www.atwoodlab.com/

 

Cell fate in development and cancer

The Atwood lab is interested in how cells choose their fate. We use models of skin and hair follicle development and skin cancer to determine how kinases control the genetic landscape of the cell to influence fate.

Cancer initiation and progression
Personalized medicine targets specific cancers by taking advantage of a tumors dependence on certain signaling pathways for growth. Mutations that activate the Hedgehog pathway drive growth of a variety of cancers including basal cell carcinoma (BCC) and medulloblastoma, accounting for up to 25% of all human cancer deaths. Despite the critical nature of Hedgehog signaling during development, how Hedgehog mediates tumor initiation, growth, and drug resistance remains poorly understood. For instance, use of drugs that target the Hedgehog pathway GPCR Smoothened are effective in treating advanced or metastatic BCC, however over 50% of advanced tumors harbor innate resistance and over 20% of tumors that initially respond to drug acquire resistance each year, illustrating the need for additional targets for therapy. Our lab has identified the oncogene atypical Protein Kinase C (aPKC) as a novel Hedgehog target gene and activator of Hedgehog signaling. aPKC forms a positive feedback loop by phosphorylating and activating the transcription factor GLI1, resulting in an increase in DNA binding and transcriptional activity. Additionally, our lab has identified that the majority of drug-resistance in BCC results from mutations in SMO that induce constitutive activity or disrupt drug interaction. Interestingly, sensitive and drug resistant BCCs magnify aPKC activity to drive high levels of pathway activation and therapeutic use of aPKC or GLI inhibitors selectively suppress Hedgehog signaling and tumor growth, suggesting the use of aPKC or GLI antagonists as viable options to treat drug-resistant tumors. Projects in the lab include determining how tumor cells regulate kinase activity, identifying novel kinase-substrate interactions that drive tumor growth, and creating novel inhibitors to target these pathways.

Stem cell fate specification
Stem cells give rise to many complex tissues such as the skin and hair follicle by controlling cell fate specification. Typically, stem cells set up a gradient of polarized components whose job is to organize intracellular signaling factors, allowing daughter cells to adopt specific fates and perform specialized functions. The conserved oncogene aPKC is found in virtually all polarized systems and serves as a master regulator of cell polarity and fate specification. A newly appreciated aspect of aPKC is the ability to phosphorylate transcription factors to regulate the genetic landscape of the cell. An open question is how posttranslational modifications control transcription factor function. Our lab has found that aPKC phosphorylates the C2H2 zinc finger domain of GLI1 to promote DNA binding and transcriptional activity, an exceedingly rare event that exerts a positive signal towards transcription factor function. Projects in the lab include exploring how phosphorylation generally regulates transcription factor activity, how kinase-transcription factor interactions specify the skin and hair follicle, and how cancers co-opt this developmental process to drive tumor growth.

See a complete list of publications at http://www.ncbi.nlm.nih.gov/pubmed?term=atwood%20sx%5BAuthor%5D

Selected publications (** Denotes equal contribution, # Denotes co-corresponding authors)

  • sm”FISH”ing for Hedgehog 
    Michael L. Drummond and Scott X. Atwood
    Journal of Investigative Dermatology. 2017. 137(1):13-5.
  • Cell-selective bioorthogonal metabolic labeling of RNA
    Kim Nguyen**, Michael Fazio**, Miles Kubota, Sarah Nainar, Chao Feng, Xiang Li, Scott X. Atwood, Timothy W. Bredy, Robert C. Spitale
    Journal of the American Chemical Society. 2017. doi:10.1021/jacs.6b11401.
  • Tumor-derived Suppressor of Fused mutations reveal Hedgehog pathway interactions
    Nicole M. Urman, Amar Mirza, Scott X. Atwood, Ramon J. Whitson, Kavita Y. Sarin, Jean Y. Tang, Anthony E. Oro
    PLoS One. 2016. 11(12):e0168031.
  • Combined treatment with arsenic trioxide and itraconazole inhibits the Hedgehog pathway in patients with refractory metastatic basal cell carcinoma: results from a pilot trial
    Mina S. Ally, Katherine Ransohoff, Kavita Sarin, Scott X. Atwood, Melika Rezaee, Irene Bailey-Healy, Jynho Kim, Philip A. Beachy, Anne Lynn S. Chang, Anthony Oro, Jean Y. Tang, A. Dimitrios Colevas
    JAMA Dermatology. 2016. 152(4):452-6.
  • Smoothened variants explain the majority of drug resistance in basal cell carcinoma
    Scott X. Atwood**, Kavita Y. Sarin**, Ramon J. Whitson, Jiang R. Li, Geurim Kim, Melika Rezaee, Mina S. Ally, Jinah Kim, Catherine Yao, Anne L.S. Chang, Anthony E. Oro#, Jean Y. Tang#
    Cancer Cell. 2015. 27(3):342-53.
  • Rolling the genetic dice: neutral and deleterious Smoothened mutations in drug-resistant basal cell carcinoma
    Scott X. Atwood, Kavita Y. Sarin, Jiang R. Li, Catherine Yao, Nicole M. Urman, Anne L.S. Chang, Jean Y. Tang, Anthony E. Oro
    Journal of Investigative Dermatology. 2015. 135(8):2138-41.
  • Advanced treatment for basal cell carcinomas
    Scott X. Atwood, Ramon J. Whitson, Anthony E. Oro
    Cold Spring Harbor Perspectives in Medicine. 2014. 4(7):a013581.
  • GLI activation by atypical protein kinase C iota/lambda regulates the growth of basal cell carcinomas
    Scott X. Atwood#, Mischa Li, Alex Lee, Jean Y. Tang, Anthony E. Oro#
    Nature. 2013. 494(7438):484-8.
  • Hedgehog pathway inhibition and the race against tumor evolution
    Scott X. Atwood, Anne L.S. Chang, Anthony E. Oro
    Journal of Cell Biology. 2012. 199(2):193-7.
  • aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division
    Scott X. Atwood and Kenneth E. Prehoda
    Current Biology. 2009. 19(9):723-9.
  • Cdc42 acts downstream of Bazooka to regulate neuroblast polarity through Par-6-aPKC
    Scott X. Atwood, Chiswili Chabu, Rhiannon R. Penkert, Chris Q. Doe, Kenneth E. Prehoda
    Journal of Cell Science. 2007. 120(Pt 18):3200-6.

Faculty Log In